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antibodies fto  (Proteintech)
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fto  (Proteintech)
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<t>FTO</t> mediates m6A demethylation of CSF3 and inhibits its expression. ( A ) The colorimetric m6A detection assay showed the overall m6A levels in CRC cells. ( B ) MeRIP combined with specific qRT-PCR was utilized to detect the relative m6A enrichment of CSF3 transcript in CCD 841 CoN and CRC cells. ( C ) Protein expression of FTO detected by western blot assay. ( D ) Transfection efficiency of oe-FTO measured in CRC cells. ( E ) qRT-PCR was utilized to detect the mRNA expression of CSF3 in CRC cells. ( F ) The colorimetric m6A detection assay showed the overall m6A levels in CRC cells after upregulation of FTO. ( G ) MeRIP combined with specific qRT-PCR was utilized to detect the relative m6A enrichment of CSF3 transcript in CCD 841 CoN and CRC cells after upregulation of FTO. ( H ) RIP assay using an anti-FTO antibody or IgG to detect the binding to CSF3 in CRC cells. IgG was used as the negative control. *compared with CCD 841 CoN or oe-NC or IgG groups. N = 3 wells per group
Fto, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fto/product/Proteintech
Average 96 stars, based on 1 article reviews
fto - by Bioz Stars, 2026-02
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anti fto  (Proteintech)
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<t>FTO</t> mediates m6A demethylation of CSF3 and inhibits its expression. ( A ) The colorimetric m6A detection assay showed the overall m6A levels in CRC cells. ( B ) MeRIP combined with specific qRT-PCR was utilized to detect the relative m6A enrichment of CSF3 transcript in CCD 841 CoN and CRC cells. ( C ) Protein expression of FTO detected by western blot assay. ( D ) Transfection efficiency of oe-FTO measured in CRC cells. ( E ) qRT-PCR was utilized to detect the mRNA expression of CSF3 in CRC cells. ( F ) The colorimetric m6A detection assay showed the overall m6A levels in CRC cells after upregulation of FTO. ( G ) MeRIP combined with specific qRT-PCR was utilized to detect the relative m6A enrichment of CSF3 transcript in CCD 841 CoN and CRC cells after upregulation of FTO. ( H ) RIP assay using an anti-FTO antibody or IgG to detect the binding to CSF3 in CRC cells. IgG was used as the negative control. *compared with CCD 841 CoN or oe-NC or IgG groups. N = 3 wells per group
Anti Fto, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti fto/product/Proteintech
Average 96 stars, based on 1 article reviews
anti fto - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier
fto antibody  (Proteintech)
96
Proteintech fto antibody
<t>FTO</t> mediates m6A demethylation of CSF3 and inhibits its expression. ( A ) The colorimetric m6A detection assay showed the overall m6A levels in CRC cells. ( B ) MeRIP combined with specific qRT-PCR was utilized to detect the relative m6A enrichment of CSF3 transcript in CCD 841 CoN and CRC cells. ( C ) Protein expression of FTO detected by western blot assay. ( D ) Transfection efficiency of oe-FTO measured in CRC cells. ( E ) qRT-PCR was utilized to detect the mRNA expression of CSF3 in CRC cells. ( F ) The colorimetric m6A detection assay showed the overall m6A levels in CRC cells after upregulation of FTO. ( G ) MeRIP combined with specific qRT-PCR was utilized to detect the relative m6A enrichment of CSF3 transcript in CCD 841 CoN and CRC cells after upregulation of FTO. ( H ) RIP assay using an anti-FTO antibody or IgG to detect the binding to CSF3 in CRC cells. IgG was used as the negative control. *compared with CCD 841 CoN or oe-NC or IgG groups. N = 3 wells per group
Fto Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fto antibody/product/Proteintech
Average 96 stars, based on 1 article reviews
fto antibody - by Bioz Stars, 2026-02
96/100 stars
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FTO mediates m6A demethylation of CSF3 and inhibits its expression. ( A ) The colorimetric m6A detection assay showed the overall m6A levels in CRC cells. ( B ) MeRIP combined with specific qRT-PCR was utilized to detect the relative m6A enrichment of CSF3 transcript in CCD 841 CoN and CRC cells. ( C ) Protein expression of FTO detected by western blot assay. ( D ) Transfection efficiency of oe-FTO measured in CRC cells. ( E ) qRT-PCR was utilized to detect the mRNA expression of CSF3 in CRC cells. ( F ) The colorimetric m6A detection assay showed the overall m6A levels in CRC cells after upregulation of FTO. ( G ) MeRIP combined with specific qRT-PCR was utilized to detect the relative m6A enrichment of CSF3 transcript in CCD 841 CoN and CRC cells after upregulation of FTO. ( H ) RIP assay using an anti-FTO antibody or IgG to detect the binding to CSF3 in CRC cells. IgG was used as the negative control. *compared with CCD 841 CoN or oe-NC or IgG groups. N = 3 wells per group

Journal: Cell Biology and Toxicology

Article Title: FTO-mediated m6A demethylation of CSF3 suppresses NETosis via downregulation of RLN2 expression in colorectal cancer

doi: 10.1007/s10565-025-10120-9

Figure Lengend Snippet: FTO mediates m6A demethylation of CSF3 and inhibits its expression. ( A ) The colorimetric m6A detection assay showed the overall m6A levels in CRC cells. ( B ) MeRIP combined with specific qRT-PCR was utilized to detect the relative m6A enrichment of CSF3 transcript in CCD 841 CoN and CRC cells. ( C ) Protein expression of FTO detected by western blot assay. ( D ) Transfection efficiency of oe-FTO measured in CRC cells. ( E ) qRT-PCR was utilized to detect the mRNA expression of CSF3 in CRC cells. ( F ) The colorimetric m6A detection assay showed the overall m6A levels in CRC cells after upregulation of FTO. ( G ) MeRIP combined with specific qRT-PCR was utilized to detect the relative m6A enrichment of CSF3 transcript in CCD 841 CoN and CRC cells after upregulation of FTO. ( H ) RIP assay using an anti-FTO antibody or IgG to detect the binding to CSF3 in CRC cells. IgG was used as the negative control. *compared with CCD 841 CoN or oe-NC or IgG groups. N = 3 wells per group

Article Snippet: Subsequently, sections were probed with primary antibodies including FTO (1:200, #27,226–1-AP, proteintech) and Ki-67 (1:1000, #28,074–1-AP, proteintech) at 4°C overnight.

Techniques: Expressing, Detection Assay, Quantitative RT-PCR, Western Blot, Transfection, Binding Assay, Negative Control